|Objective || |
To determine the concentration of ethanol in a blood sample; to see if the person being tested was legally drunk.
|Sample || |
A simulated blood plasma with added ethanol. Store the aqueous solution in a tightly closed vial in the refrigerator; the solution is stable for at least one month.
|Ethanol (analyte), n-propanol (internal standard) |
Standard Reference Material
The standard reference material is simulated blood plasma with a concentration of 0.10% (w/v) ethanol. Store the aqueous solution in a tightly closed vial in the refrigerator; the solution is stable for at least one month.
|Gas chromatography; direct aqueous injection |
|Special equipment || |
Capillary gas chromatograph with thick-film Carbowax column (e.g. 30 m x 0.25 mm x 0.5 µm) and flame ionization detector
For instructor (detailed) ; For student (brief)
|Typical results || |
Chromatogram: 1 µL injection of liquid sample at 1:40 split; isothermal separation at 70 degrees C; 1 mL/min helium flow; elution order is (1) ethanol (2) propanol
Calibration curve: (peak area ethanol / peak area propanol) vs. concentration of ethanol
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